Dye tracing is tracing and tracking various types of flow using added dye to certain liquids. The purpose of doing this is to analyze the flowing in itself or the way a flow transports something as it conveys the flow. The float tracing method which has been around for ages has evolved into what is known as dye tracing. This consists basically of throwing a floating object into a flow of water to see where it will eventually emerge or end up. Tracking dye may be quantitative or qualitative. It can be quantitative when special instruments are used for tracing dye. It can be qualitative when the estimate and the presence of a flow is tracked.
Dye Tracing Applications
When is dye tracing used? Dye tracing is used for analyzing the circulation of blood within various parts of the animal as well as the human body. For instance, one analysis technique for retina circulation is called fluorescent angiography which is used for the analysis of various diseases of the eyes. With current developments in dye tracing techniques, it is possible to track single cell migrations tagged by fluorescent molecules of single cells. For instance, cells activated with fluorescence make it possible to detect which cells are flowing where sorted out through flow cytometry.
Typically, applications of dyed water flow also include the analysis of storm water and sewer drainage systems. It also includes analyzing natural water flow in the environment such as in groundwater filtration, karst studies, cave water flows, ocean currents, lakes and rivers. Pollution studies are also another application where tracing is used. Leak detection and piping or plumbing tracing is another application.
Methods of Dye Tracing
The environment differentiates water flows and these possess specific factors affecting the performance of a dye. In a flow of water, natural fluorescence interferes with specific dyes. The presence of chemicals, organics and sunlight is able to influence a dye’s intensity. Each sample area is analyzed by instruments that are quantitative to test fluorescence backgrounds. Every dye you use has significant factors of performance distinguishing it in various set ups. These aspects are influential in terms of how each of the dyes interact and are affected by certain environments. Thus it can sometimes be particularly useful for one dye to be in a certain environment but not in another. Factors that might affect performance include limitations of using these in waters that are acidic, surface loss of water and absorption resistance.
Quantitative Dye Tracing
The first ever technology-assisted tracing using dye was called carbon sampling. This was based on how charcoal absorbed dye. Packets of charcoal can be arranged along the flow’s expected route. The collected dye can late be extracted chemically for an evaluation done on the amount. The first device that was able to detect concentration of dyes beyond what the human eye was able to see was called a filter fluorometer. Later, the analysis of fluorescence was enhanced by the development of spectrofluorometers in the eighties. Both spectrofluorometers and fluorometers were able to identify fluorescence intensity when liquid samples were present. Chemicals and different dyes produce a certain wavelength that is measured through analyzing methods.